Were in complete agreement with the results of the culture method the sensitivity and specificity for both qpcr assays were 100 when using Dna extracted by boiling or instagene methods from 24h incubated mbpw pre enrich ment broth the next best extraction method was the prepseq auto dna extraction made from 1000 ml of pre enrichment cultures performing pre pseq auto dna extraction from 300 ml pre enrichment culture caused from pine nuts using the culture