The results of the present study confirmed the harmful effects of X-rays on human DNA. These radiations-imposed genotoxic effects can cause significant DNA damage in radiology personnel during their occupational exposure. The increase in DNA damage may be attributed to handling of X-ray examinations without the use of any protective measures; therefore, the DNA damage in the workers using a lead apron was lower than in those who did not use a lead apron. Thus radiation personnel need to be aware about the hazards associated with excessive exposure to radiation.