2.8 Determination of the Minimum Bactericidal
Concentration (MBC)
The tubes showed no apparent growth of microorganism
in the MIC assay was subcultured on a BHI agar
(Difco) plates by making streak the surface of agar, and
incubated at 37oC for 24 hours. Least concentration of
extracts showing no visible growth on new agar medium
was taken as MBC.