The samples were milled for further analysis. 1 g of
ground plant material with 100 mL of pure methanol was
extracted for 1 h, using an ultrasonic bath Branson model b-220
Smith-Kline Company (50/60 Hz, 125 W). The procedure of
extraction was similar to that used by (Škerget et al., 2005) but
applied extraction period was 1 hour shorter. The temperature
of extraction was 40 oC at atmospheric pressure. The solvent
was evaporated under vacuum using a rotary evaporator and
the yield of extraction was determined in wt%. The extracts
were stored in a dry and cool place