The film dried standards were, as indicated by the manufacturer, reconstituted in 5 mL of solvent (acetonitrile), to give certified concentrations of 100.0 μg/mL (uncertainty: ±5.0 μg/mL) and of 25.0 μg/mL (uncertainty: ±1.5 μg/mL), respectively for the main ergot alkloids and for the -inine isomers