Purpose : This work focuses on the eff ect of gamma radiation
on seed born fungi (in vitro) on Oryza sativa (Swarna, Initial
Evaluation Trial-5656).
Materials and methods : The responses of fungi to gamma
radiation (0 – 4.2 kGy; 0.12 kGy/h) were studied in individual
cultures of major seed-borne fungi including Alternaria
alternata , Aspergillus fl avus , Trichoderma viride and Curvularia
geniculata .
Results : The inactivation of individual fungal-viability was
noted between 1.0 – 2.0 kGy for A. alternata and A. fl avus and
0.5 – 1.0 kGy for T. viride and C. geniculata . Complete inhibition
was observed at 2.5 kGy. Formations of multiple germ tubes
were noted in A. alternata and A. fl avus at 2 kGy and 2.5 kGy,
respectively. A. fl avus required a higher dose to reduce viability
to 10% (D 10 ) value in comparison to other selected fungi.
Conclusions : Diff erent fungi exhibited diff erent radiosensitivity.
The dose range of 2 – 2.5 kGy was eff ective in killing all selected
fungi. The fungi showing a higher D 10 value exhibited multiple
germ tubes.
Keywords: γ -ray , fungi , morphology
Purpose : This work focuses on the eff ect of gamma radiationon seed born fungi (in vitro) on Oryza sativa (Swarna, InitialEvaluation Trial-5656).Materials and methods : The responses of fungi to gammaradiation (0 – 4.2 kGy; 0.12 kGy/h) were studied in individualcultures of major seed-borne fungi including Alternariaalternata , Aspergillus fl avus , Trichoderma viride and Curvulariageniculata .Results : The inactivation of individual fungal-viability wasnoted between 1.0 – 2.0 kGy for A. alternata and A. fl avus and0.5 – 1.0 kGy for T. viride and C. geniculata . Complete inhibitionwas observed at 2.5 kGy. Formations of multiple germ tubeswere noted in A. alternata and A. fl avus at 2 kGy and 2.5 kGy,respectively. A. fl avus required a higher dose to reduce viabilityto 10% (D 10 ) value in comparison to other selected fungi.Conclusions : Diff erent fungi exhibited diff erent radiosensitivity.The dose range of 2 – 2.5 kGy was eff ective in killing all selectedfungi. The fungi showing a higher D 10 value exhibited multiplegerm tubes.Keywords: γ -ray , fungi , morphology
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