White spot syndrome virus is currently the leading cause of production losses in the shrimp industry.
Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protective
effect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve as
an alternative source for in-feed vaccination, considering the remarkable abilities of plant expression
systems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinant
PmRab7 showed high binding activity against white spot syndrome virus and a viral envelope,
VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembled
that of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5 mg g−1 dry
weight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Coinjection
of white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeus
vannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we propose
an alternative PmRab7 source with higher production yield, and cheaper culture media costs, that
might serve the industry’s need for an in-feed supplement against white spot syndrome infection.
© 2012 Elsevier B.V. All rights reserved.
White spot syndrome virus is currently the leading cause of production losses in the shrimp industry.Penaeus monodon Rab7 protein has been recognized as a viral-binding protein with an efficient protectiveeffect against white spot syndrome infection. Plant-derived recombinant PmRab7 might serve asan alternative source for in-feed vaccination, considering the remarkable abilities of plant expressionsystems. PmRab7 was introduced into the Arabidopsis thaliana T87 genome. Arabidopsis-derived recombinantPmRab7 showed high binding activity against white spot syndrome virus and a viral envelope,VP28. The growth profile of Arabidopsis suspension culture expressing PmRab7 (ECR21# 35) resembledthat of its counterpart. PmRab7 expression in ECR21# 35 reached its maximum level at 5 mg g−1 dryweight in 12 days, which was higher than those previously reported in Escherichia coli and in Pichia. Coinjectionof white spot syndrome virus and Arabidopsis crude extract containing PmRab7 in Litopenaeusvannamei showed an 87% increase in shrimp survival rate at 5 day after injection. In this study, we proposean alternative PmRab7 source with higher production yield, and cheaper culture media costs, thatmight serve the industry’s need for an in-feed supplement against white spot syndrome infection.© 2012 Elsevier B.V. All rights reserved.
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