High-yielding rice was harvested in Hyogo prefecture, Japan. The rice was either left in its harvested state or polished before being ground to produce flour with a particle size of approximately 0.5 mm using a laboratory disintegrator for use as carbon sources for ethanol fermentation. According to the analysis of the Hyogo Prefectural Technology Center for Agriculture, Forestry and Fisheries, the high-yielding brown rice was composed of 12.8% water, 75.3% carbohydrate, 8.3% protein, 2.3% lipid, and 1.3% ash.