finely ground in a mortar, and extracted with
solvent in a ratio of 1:10 (w/v) for 24 h at room temperature. The mixture was then
centrifuged at 13,000 rpm for 10 min at room temperature and the supernatant
collected for filtration. For aqueous extracts, the filtrates were frozen at−80 °C for 48 h
before being freeze-dried. For the other extracts, the filtrates were evaporated to
dryness in a rotary evaporator before being frozen at −80 °C for 48 h prior to being
freeze-dried