Crude protein mixtures are put in contact with the
adsorbent by using the appropriate conditions to
selectively bind the enzyme, while nearly all other
proteins are not adsorbed and are removed by
washing with buffer solution (unbound fractions).
A subsequent additional resolution step is obtained
by gradually reducing the strength by which proteins
are adsorbed on the support, so obtaining the sequential
elution of the fractions (different lipases and/or
contaminating proteins). With this system it was also
possible to hypothesize a partial resolution of different
isoforms. All the fractions obtained were characterized
by different electrophoretic methods.