The fish were randomly divided into 4 experimental groups
with three replicates in each. Tank capacitywas 200 L and each tank
contained 15 fish. Fish were fed one of 4 diets (Control, D-I, D-II, or
D-III). The feed rate was 3% of body weight per day, and equal
rations were provided at 09.00 and 17.00 h for 60 days. The amount
of diet consumed was determined by daily recovery of excess feed,
which was then dried and weighed [20]. Daily feed was adjusted
every 15 days by batch weighing after 24 h of starvation.