Sixty-three phytase-producing bacterial strains were isolated from natural sources, and their probiotic
potential was evaluated. Of these, only fifteen strains were selected on the basis of confirmatory plate
assay. Among these, five phytase-producing strains exhibiting potent probiotic properties were identified
as Bacillus cereus P1, Bacillus subtilis P6, B. subtilis P7, Pseudomonas aeruginosa P12 and P. aeruginosa
P15 on the basis of morphological, biochemical and molecular characteristics. Maximum phytase activity
(2.74 EU mL
1) with potential probiotic properties, that is more than 70% survivability at pH 2.0, up to
2.0% bile salt tolerance, sporulation efficiency of more than 80% and survival in anaerobic condition
(94.31%), was revealed by B. subtilis P6 as compared to the well-established commercial probiotic strains
Lactobacillus sporogenes and Lactobacillus casei. Thus, phytase-producing B. subtilis P6 with promising
probiotic features can be used in food and animal feed applications for betterment of mankind after further
validation.
Sixty-three phytase-producing bacterial strains were isolated from natural sources, and their probioticpotential was evaluated. Of these, only fifteen strains were selected on the basis of confirmatory plateassay. Among these, five phytase-producing strains exhibiting potent probiotic properties were identifiedas Bacillus cereus P1, Bacillus subtilis P6, B. subtilis P7, Pseudomonas aeruginosa P12 and P. aeruginosaP15 on the basis of morphological, biochemical and molecular characteristics. Maximum phytase activity(2.74 EU mL1) with potential probiotic properties, that is more than 70% survivability at pH 2.0, up to2.0% bile salt tolerance, sporulation efficiency of more than 80% and survival in anaerobic condition(94.31%), was revealed by B. subtilis P6 as compared to the well-established commercial probiotic strainsLactobacillus sporogenes and Lactobacillus casei. Thus, phytase-producing B. subtilis P6 with promisingprobiotic features can be used in food and animal feed applications for betterment of mankind after furthervalidation.
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