The experiment was run in a set of 10 flasks, each carries 100mg
of PCL film piece in 90 mL MSM and inoculated with 10 mL (v/v) of
24 h old culture. The flasks were incubated in an orbital shaker
incubator at 50 C and 150 rpm for 10 days. Both biotic (without PCL
film piece) and abiotic (without bacterial cells) controls were run in
parallel with test. The experiment was run in triplicate. Each set of
flask was used for data collection (cfu, OD600, and measurement of
weight loss) at 24 h interval. The growth in test vessel was checked
by measuring cfu/mL and optical density at 600 nm using spectrophotometer
and compared with that of biotic control, finally a
growth curve was plotted.