The selective bioassays were performed on fresh artificial diet comprising 17 g agar (Biobra´s, Brazil), 42.9 g brewer’s yeast, 67.4 g wheat germ, 140.4 g minced beans, 4.3 g ascorbic acid in a total volume of 1.0 l, poured into
a 24 well cell culture plate (1.5 ml per well). After solidification, 35 ll of the resuspended spore/crystal powder was
applied onto the diet surface in each well and allowed to dry.