If no CPE occurs, the test may be declared negative.
4.3.1.2.1.2 Identification of RSIV and ISKNV
RSIV (probably ISKNV also) cannot be identified by neutralisation tests as the antisera generated by
the immunisation of rabbits have few neutralising antibodies.
4.3.1.2.1.2.1 Indirect fluorescent antibody test
Samples to be taken for testing include acetone-fixed infected cell monolayers that have developed
CPE.
Use an uninfected cell monolayer as a negative control, and use a RSIV-infected cell monolayer as a
positive control.