Our P. stylirostris samples collected from Brunei in 2006
also showed the presence of microsporidium spores within the
hepatopancreas (Fig. 1C), where the tubule epithelial cells had a
positive reaction by ISH (Fig. 1D). In both cases, the hepatopancreas
was the only tissue that reacted to the EHP probe, and the
ISH results were associated with lesions detected by H&E
histology. These results are similar to those described by
Tangprasittipap et al. (2013) with samples of EHP-infected
P. vannamei. The diagnosis of EHP in the samples from Brunei also
show that EHP has been present in shrimp farms in Asia at least
since 2006, earlier than previously thought.