Briefly, Cancer cells were
serum-starved overnight and then pre-treated with GA in
6-well plates for 24h. The monolayers were then scratched
with a 200μl sterile pipette tip. Floating cells were washed
off with PBS, and the adherent cells were maintained in
serum-free media. Images of the same fields were acquired
by microscope (Nikon Instruments Inc.) at a magnification
of 100× at two preselected time points (0 h and 24 h).