In order to confirm our hypotheses that the high concentration
of sucrose present in green coffee beans was counteracting the
antibacterial effect of natural coffee compounds, we performed
an additional experiment incubating the S. mutans inoculum in test
tubes containing plain medium (control) or the same medium supplemented
with sucrose. The concentration of sucrose used in this
test was similar to that found in the C. canephora sample from
Group 1. The number of CFU from the control tube was
1.2 106, while the number of CFU of the supplemented medium
tube was 5.3 106, 4.4 times higher than in non-supplemented
media. This result increases evidence that sucrose may counteract
the antibacterial effect of natural occurring compounds in green
coffee beans.