bufferedpeptone and was properly blended.
1 mL dilutions were inoculated ontoTPC petrifilm (PetrifilmR 3M) to obtain Total Plate Count and incubated at30Cfor 48h.
Furthermore, 1 ml dilutions were inoculated on to E coli petrifilm (3M™ Petrifilm E coli/Coliform count plates) and Baired Parker medium (Oxoid, UK) to obtain E. coli and S. aureus count respectively at 37 C for 24 h.