Microbiological analyses
Preparation of decimal dilutions of VJs, Ins and KLBs was in Ringer's solution (Sigma–Aldrich, Milan, Italy). The cell suspensions were used to estimate the following microbial groups: total mesophilic count (TMC) on plate count agar (PCA), incubated aerobically at 30 °C for 72 h; Enterobacteriaceae on double-layered violet red bile glucose agar (VRBGA), incubated aerobically at 37 °C for 24 h; pseudomonads on Pseudomonas agar base (PAB) supplemented with 10 mg/mL cetrimide fucidin, incubated aerobically at 20 °C for 48 h; rod LAB on de Man-Rogosa-Sharpe (MRS) agar, acidified to pH 5.4 with lactic acid (5 mol/L) and incubated anaerobically at 30 °C for 48 h; coccus LAB on M17 agar, incubated anaerobically at 30 °C for 48 h; yeasts on dichloran rose Bengal chloramphenicol (DRBC) agar, incubated aerobically at 25 °C for 48 h. All media and supplements were purchased from Oxoid (Milan, Italy). Count plates were carried out in duplicate for each independent production.
Microbiological analysesPreparation of decimal dilutions of VJs, Ins and KLBs was in Ringer's solution (Sigma–Aldrich, Milan, Italy). The cell suspensions were used to estimate the following microbial groups: total mesophilic count (TMC) on plate count agar (PCA), incubated aerobically at 30 °C for 72 h; Enterobacteriaceae on double-layered violet red bile glucose agar (VRBGA), incubated aerobically at 37 °C for 24 h; pseudomonads on Pseudomonas agar base (PAB) supplemented with 10 mg/mL cetrimide fucidin, incubated aerobically at 20 °C for 48 h; rod LAB on de Man-Rogosa-Sharpe (MRS) agar, acidified to pH 5.4 with lactic acid (5 mol/L) and incubated anaerobically at 30 °C for 48 h; coccus LAB on M17 agar, incubated anaerobically at 30 °C for 48 h; yeasts on dichloran rose Bengal chloramphenicol (DRBC) agar, incubated aerobically at 25 °C for 48 h. All media and supplements were purchased from Oxoid (Milan, Italy). Count plates were carried out in duplicate for each independent production.
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