Corona Discharge Method
Plasma has been used for seed mutation. Atmospheric plasma discharge equipment was used. It had two parallel
high voltage electrodes. Seeds were put under atmospheric pressure plasma, the plasma would bring a mass
electron, ion and ozone, and the mass electrons were faster. The ozone was the main component to react on tomato
seed for mutation. Various trials were carried out and operating voltage levels as 2 kV, 4 kV and 6 kV
were fixed with time interval 15, 10, 5 second at high frequency of 15 kHz.
For these all methods, germination tests were carried out. For each method nine variable combinations of voltage
and time were considered. One untreated seed lot was used as control. Each seed lot was of 50 seeds. Three
replications were utilized for statistical analysis. Germination trials were carried out in seed germinator where
temperature and humidity was maintained at 25˚C at 80% relative humidity. Similarly same seeds were also sown
in the plugs filled with soil having sufficient moisture well suited as open field conditions. Germination percentage
was calculated as per standards. Additionally, root shoot length and seed vigor study was also completed