Continuing loss of wild orchid habitat in Thailand has led to an increased emphasis on orchid conservation. Major obstacles in the production of wild orchid seedlings for use in conservation have been development of efficient media on seed germination and protocorm development. Effects of seven asymbiotic media (Modified Vacin & Went) on seed germination and five asymbiotic media ( Modified Vacin & Went) on protocorm development of Arundina graminifolia were examined. After three months culture, percent seed germination was highest on Modified Vacin & Went supplement with Ear mushroom and 10 g/L activated charcoal (82.50%). However, there are no significant differences in statistical with 99% on five asymbiotic media (Modified Vacin & Went) for early protocorm development of Arundina graminifolia after ten weeks culture.