To investigate the tumor cytotoxicity and cytokines production of plenocytes In the mice immunized by the splenocytes were separated and mix H22 tunur cells ity of splenocytes and the levels of ll 2, IFN-y, IL-10 and Il 4 in the supernatant were measured. The results show that the cytotoxicity to H22 tumor cells of the NTCSM-immunized group was higher than that of the sterile saline-injected group and untreated macrophage- vaccinated group(p 0.05), Meanwhile, the Tevels of IL2 and lFN-ry in the culture supernatant of the NTcsM-immunized group were obvi ously higher than those of the saline-injected group and untreated macrophage-vaccinated group, but the level of IL-4 of the NTCSM immunized group was lower than those of the other two groups