Biological urban water treatment plants utilize microorganisms for wastewater purification so that
microbiological characterization of processes is very important. In addition, its removal in the effluent
and outlet sludge for their reuse is important. This work aims to characterize the presence of bacteria and
parasites along the processes in wastewater treatment plant (WWTP) and the possibility of using the
purifying water and sludge in agriculture.
The results show a high level of Escherichia coli in the raw water. Although their total removal is not
achieved, a reduction of 2.34 and 1.36 log in the concentration of E. coli was produced along the water and
sludge treatment lines, respectively, being the trickling filters (TF) and autothermal thermophilic aerobic
digestion (ATAD) the most effective processes against bacteria.
Clostridium perfringens, which is a Grampositive bacillus and fecal contamination indicator, although
less usual than E.coli, is detected in washing water of solids which are stored in anoxic conditions and in
the sludge treatment line where dissolved oxygen is absent including in the outlet of plant.
Salmonellaspp,Entamoebaand Cryptosporidiumwere not detected in any of the samples, meanwhile
Giardia duodenaliswas identified only in two samples from washing coarse solids and sludge, but it was
not identified in outlet water and sludge. Acanthamoebawas the most frequent protozoa isolated.
Biological urban water treatment plants utilize microorganisms for wastewater purification so thatmicrobiological characterization of processes is very important. In addition, its removal in the effluentand outlet sludge for their reuse is important. This work aims to characterize the presence of bacteria andparasites along the processes in wastewater treatment plant (WWTP) and the possibility of using thepurifying water and sludge in agriculture.The results show a high level of Escherichia coli in the raw water. Although their total removal is notachieved, a reduction of 2.34 and 1.36 log in the concentration of E. coli was produced along the water andsludge treatment lines, respectively, being the trickling filters (TF) and autothermal thermophilic aerobicdigestion (ATAD) the most effective processes against bacteria.Clostridium perfringens, which is a Grampositive bacillus and fecal contamination indicator, althoughless usual than E.coli, is detected in washing water of solids which are stored in anoxic conditions and inthe sludge treatment line where dissolved oxygen is absent including in the outlet of plant.Salmonellaspp,Entamoebaand Cryptosporidiumwere not detected in any of the samples, meanwhileGiardia duodenaliswas identified only in two samples from washing coarse solids and sludge, but it wasnot identified in outlet water and sludge. Acanthamoebawas the most frequent protozoa isolated.
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