Three mature cattle bulls were used for semen collection. Semen samples were diluted with TRIS extender
(control) and TRIS-Honey bee extender (with a concentration of 0.5/4.5, 1/4, 1.5/3.5, 2/3, 2.5/2.5 ml (v/v) of honey
/ TCFY to obtain a final volume of 5 ml in each tube). Extended semen was slowly cooled, packed into 0.25 ml
straws and frozen in liquid nitrogen. The parameters studied were subjective semen characteristics
(motility, alive, abnormality and hypoosmotic swelling test (HOST) %) with the conception rate as a
confirmatory field test. Results revealed that sperm motility % of the concentration 1.5/3.5 cc was the best after
7 days of chilling (55.55%), while 0.5/4.5 cc concentration was the best after thawing (62.50%) with a conception
rate of 82.35%. Finally, it is concluded that, addition of 10% honey solution to semen extender improved sperm
motility in chilled and frozen semen and also improved the conception rate.
Key words:
Three mature cattle bulls were used for semen collection. Semen samples were diluted with TRIS extender(control) and TRIS-Honey bee extender (with a concentration of 0.5/4.5, 1/4, 1.5/3.5, 2/3, 2.5/2.5 ml (v/v) of honey/ TCFY to obtain a final volume of 5 ml in each tube). Extended semen was slowly cooled, packed into 0.25 mlstraws and frozen in liquid nitrogen. The parameters studied were subjective semen characteristics(motility, alive, abnormality and hypoosmotic swelling test (HOST) %) with the conception rate as aconfirmatory field test. Results revealed that sperm motility % of the concentration 1.5/3.5 cc was the best after7 days of chilling (55.55%), while 0.5/4.5 cc concentration was the best after thawing (62.50%) with a conceptionrate of 82.35%. Finally, it is concluded that, addition of 10% honey solution to semen extender improved spermmotility in chilled and frozen semen and also improved the conception rate.Key words:
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