As expected, epoxy-carotenoids and hydroxy-carotenoids, such
as violaxanthin and lutein, were the first to elute in the reverse
phase column, followed by f-carotene, a-carotene, all-trans-bcarotene
and cis-b-carotene, respectively. Peak 3 was not identified.
Peaks 4 and 5 showed chromatographic data and UV–visible
absorption spectra similar to those described for the carotenoids
zeaxanthin and a-cryptoxanthin, respectively, as had already been
noted in another study involving the same species of pumpkins
(Azevedo-Meleiro & Rodriguez-Amaya, 2007). However, because
they are present in low concentrations, it was not possible to obtain
isolation by OCC, therefore the spectra were not determined in
other solvent systems nor were the necessary reactions of identification
carried out, and thus only one indication of the identity of
those carotenoids was considered. Other minor peaks were also
ignored. Typically, one to four carotenoids are predominant in the
pumpkin species, with several other compounds detected in low
concentrations or traces. The separation, identification, and quantification
of these carotenoids were not the aim of this work; they can
be better studied with the use of a mass spectrophotometer
(Azevedo-Meleiro & Rodriguez-Amaya, 2004).
As expected, epoxy-carotenoids and hydroxy-carotenoids, suchas violaxanthin and lutein, were the first to elute in the reversephase column, followed by f-carotene, a-carotene, all-trans-bcaroteneand cis-b-carotene, respectively. Peak 3 was not identified.Peaks 4 and 5 showed chromatographic data and UV–visibleabsorption spectra similar to those described for the carotenoidszeaxanthin and a-cryptoxanthin, respectively, as had already beennoted in another study involving the same species of pumpkins(Azevedo-Meleiro & Rodriguez-Amaya, 2007). However, becausethey are present in low concentrations, it was not possible to obtainisolation by OCC, therefore the spectra were not determined inother solvent systems nor were the necessary reactions of identificationcarried out, and thus only one indication of the identity ofthose carotenoids was considered. Other minor peaks were alsoignored. Typically, one to four carotenoids are predominant in thepumpkin species, with several other compounds detected in lowconcentrations or traces. The separation, identification, and quantificationof these carotenoids were not the aim of this work; they canbe better studied with the use of a mass spectrophotometer(Azevedo-Meleiro & Rodriguez-Amaya, 2004).
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