Honeydew assays. For quantitative analysis of honeydew, modified Parafilm sachet
technique was used49. A Parafilm sachet was attached to the leaf sheath of each
seedling at the 5– to 6-leaf stage. A female BPH within 2 days after emergence was
then enclosed in a Parafilm sachet. After 48 h of incubation at 25uC under a 16-h
light:8-h dark cycle, each insect was removed, and the sachet was weighed before and
after the removal of honeydew. Each line of plants was tested 10 times by using fresh
insects and fresh plants. The data was analyzed using Tukey–Kramer test28.