A kinetic study on partial hydrolysis for the production of diacylglycerol (DAG) catalyzed by Rhizomucor
meihei (Lipase RM IM) was investigated. The optimum conditions were estimated to be 10% (w/w) catalyst
with 5% (w/w) water content at 55 ◦C with stirring speed of 500 rpm based on the initial reaction
velocity of 7.04 mmol/L/min. At optimum conditions, the DAG yield was 31 wt% after 6 h of reaction. The
rheological properties of both untreated palm oil and DAG-enriched palm oil were also investigated and
both exhibited Newtonian flow behavior with insignificant differences with respect to their viscosity
and flow activation energy. Activation energy for the partial hydrolysis of palm oil was calculated to be
31.35 kJ/mol using Arrhenius equation. Morphological evaluation of enzyme particles revealed that high
speed rate might devastate the enzyme particles as those particles were susceptible to mechanical force.
A ping-pong bi-bi with substrate competitive inhibition by water kinetic model had been proposed to
simulate the reaction kinetic of partial hydrolysis. The kinetic constants Vmax, Km (palm oil), Km (water) and Ki
were evaluated to be 17.39 mmol/L/min, 0.55 mmol/L, 1.04 mmol/L and 2.68 mmol/L, respectively using
non linear regression analysis with the computer program Microsoft Excel Solver. The model proposed
was found to accurately represent the experimental data with determination coefficient R2 of 0.99.
A kinetic study on partial hydrolysis for the production of diacylglycerol (DAG) catalyzed by Rhizomucormeihei (Lipase RM IM) was investigated. The optimum conditions were estimated to be 10% (w/w) catalystwith 5% (w/w) water content at 55 ◦C with stirring speed of 500 rpm based on the initial reactionvelocity of 7.04 mmol/L/min. At optimum conditions, the DAG yield was 31 wt% after 6 h of reaction. Therheological properties of both untreated palm oil and DAG-enriched palm oil were also investigated andboth exhibited Newtonian flow behavior with insignificant differences with respect to their viscosityand flow activation energy. Activation energy for the partial hydrolysis of palm oil was calculated to be31.35 kJ/mol using Arrhenius equation. Morphological evaluation of enzyme particles revealed that highspeed rate might devastate the enzyme particles as those particles were susceptible to mechanical force.A ping-pong bi-bi with substrate competitive inhibition by water kinetic model had been proposed tosimulate the reaction kinetic of partial hydrolysis. The kinetic constants Vmax, Km (palm oil), Km (water) and Kiwere evaluated to be 17.39 mmol/L/min, 0.55 mmol/L, 1.04 mmol/L and 2.68 mmol/L, respectively usingnon linear regression analysis with the computer program Microsoft Excel Solver. The model proposedwas found to accurately represent the experimental data with determination coefficient R2 of 0.99.
การแปล กรุณารอสักครู่..