Hydrocarbon utilizing fungi were enumerated by viable plate count, using spread plate technique. Bushnell Hass Agar supplemented with 50ug/ml of streptomycin was prepared and pH adjusted to 5.8, then 0.1ml of 10-3 and 10-4 dilutions were inoculated unto the medium in duplicate, at same time sterile whattman No. 1 filter paper was
saturated with 1ml of crude oil and place aseptically on the lid of the inoculated plate (Atlas and Bartha). The plates were taped round with masking tape. This was done to provide the crude oil as the soul source of carbon and energy for the growth of the organisms on the surface through vapour phase transfer. The plates were then incubated at room temperature for 4-7 days after which fungal count was recovered.