2.6.1.3. Determination of acid value (AV). Acid values (AV) were estimated according to AOAC (2005). Acid value was analyzed by titration of approximately 0.5 g of lipid from Soxhlet extraction, dissolved in a mixture of 100 ml of ethanol and diethyl ether (1:1; v/v), with 0.01 N potassium hydroxide. Phenolphthalein was used as indicator. The results were expressed as mg KOH/g lipid.
ml of Alkali N 56:1
Acid value ðmg of KOH=g of sampleÞ ¼ ð Þ
Wt : of sample in g