Separation of biomass is a big challenge in SSF, which is essential
for the kinetic studies. However certain indirect methods are
there such as, glucosamine estimation, ergosterol estimation, protein
(kjeldahl) estimation, DNA estimation, dryweight changes and
CO2 evolution, but all of them have their ownweaknesses. Recently
digital image processing has been developed as a tool for measuring
biomass in SSF. The images were acquired by stereomicroscope
and a digital camera and processed using KS400 software [12]. In
recent times estimation of oxygen intake and carbon dioxide evolution
rate are considered to be most accurate for the determination
of growth of the microorganism