The working FRAP reagent was prepared daily by mixing 10 volumes of 300 mM acetate buffer, pH 3.6, with 1 volume of 10 mM TPTZ (2,4,6-tri(2-pyridyl)-s-triazine) in 40 mM hydrochloric acid and with 1 volume of 20 mM ferric chloride
The working FRAP reagent wasprepared daily by mixing 10 volumes of 300 mM acetate buffer, pH3.6, with 1 volume of 10 mM TPTZ (2,4,6-tri(2-pyridyl)-s-triazine) in40 mM hydrochloric acid and with 1 volume of 20 mM ferric chloride