3. RESULT AND DISCUSSION
Shoot tip explants of 2 cm each were cultured
on the MS media supplemented with different
concentrations of BA (2.22 to 0.44) and IBA (4.9 to
o.44). The callus formation including Plant regeneration
showed better response within 6 weeks of culture in
medium 2.22 μM/L BA and 4.9 μM/L IBA compared to
other media combinations applied. Higher number of
multiple shoot induction was observed in 8 weeks with
higher shoot, petiole and leaf length (Table 2) in the
above mentioned concentration. This result also confirm
the findings of Kumari et al., 2008, Nahar & Borna,
2012 and Rajore et al., 2007,those who demonstrated the
effect of growth regulators on the callus formation and
also multiple shoot formation from the callus of Ricinus
communis and Jatropha curcas respectively.