RAW 264.7 murine macrophages were obtained from American Type Culture
Collection (ATCC, Rockville, MD, USA). These cells were cultured in Dulbecco’s Modified
Eagle’s Medium (DMEM) supplemented with 10% FBS, 100 U/mL penicillin, and
100 lg/mL streptomycin at 37 C under 5% CO2-humidified air.