The present work demonstrated that the self-directing optimization
technique was an efficient tool for the optimization
of submerged culture conditions for mycelial biomass
and EPS production by G. frondosa. It was found that the
culture pH, aeration rate, and hydrodynamic behavior led
to significant differences in the mycelial morphology of G.
frondosa and subsequently affected EPS yields. In G. frondosa
fermentation, the stirred-tank fermenter was considered
more efficient than airlift fermenter, in that higher yields of
mycelial biomass and EPS were achieved. The results obtained
are considered useful for the production of Grifola
polysaccharides on a large scale and can be widely applied
to other mushroom fermentations.