grown in 15-liter plastic pots under greenhouse conditions and at
anthesis, stigmas were pollinated. Subsequently, 10 μl of a suspension
containing ≈1 × 108 AAC00-1 CFU/ml (≈1 × 106 CFU/
blossom) was transferred to each stigma using a micropipette.
Five blossoms were inoculated per plant, tagged, and allowed to
develop for 30 days after pollination. Thirty-five to forty fruits
were harvested at maturity and seeds were extracted as described
above and pooled to a produce each seed lot