Production of biomass using S. dimorphus was successfully carried out where 1.25 g/L of dry biomass was obtained after 12 h of natural sedimentation. The dried biomass produced consists of 14 ± 0.6% w/w lipid and 36 ± 0.5% w/w starch (Table 1). Solvent extraction of the fresh biomass offered lipid for the production of biodiesel and the residual biomass for the production of bioethanol (Fig. 1). GC-FID analysis has shown lipid extracted from S. dimorphus had the potential to be converted into biodiesel, since it composed mainly of methyl palmitate, methyl stearate and methyl oleate. The summary of major fatty acids based on the chromatogram peaks were tabulated in Table 2. The highest percentage belongs to saturated palmitic acid which was about 33.89%. While the second largest fatty acid is belongs to the unsaturated oleic acid with a percentage of 21.91%. The chromatogram also indicates the presence of many other fatty acids but at a much lower percentage for example, the palmitoleic acid and stearic acid at an average percentage of 3%. The conversion yield of these fatty acids into