Colour was determined in the equatorial perimeter at two
opposite fruit zones by using a Minolta colorimeter
(CRC200, Minolta Camera Co., Japan) and expressed as
Hue angle.
For each sample, the juice was obtained by choking with
a mortar and pestle and filtrated with cheese cloth. TSS
were measured in duplicate with a digital refractometer,
Atago PR-101 (Atago Co. Ltd., Japan), at 20 °C, and
results are the means±SE expressed in percentage (°Brix).
The pH of the juice was recorded, and then TA was
determined in duplicate by potentiometric titration with
0.1 N NaOH up to pH 8.1, using 1 mL of diluted juice in
25 mL distilled H2O, and results are the means±SE
expressed as grammes of malic acid equivalent per 100 g-1
fresh weight (FW).