the oven temperature was programmed from 100 to 220 ◦C at a
rate of 10 ◦C/min. The carrier gas flow rate was 3.4 mL/min, and
the split ratio was 50 : 1.
The unsaponifiable matters of egg yolk were determined by following AOCS Official Method Ca 6b-53 using 5 g of yolk sample per replicate.