Interestingly, it was recently demonstrated that addition of a mixture of 20 amino-acids or a yeast extract greatly improved the fermentation efficiency of K. africana (the imperfect state of H. vineae) compared with tequila fermentations supplemented with ammonium sulfate alone (Díaz-Montaño et al., 2010; Valle-Rodríguez et al., 2012). Literature in reference to the vitamin sufficiency effec