The SPC and SPC:SA systems showed a significant decrease in the %EE from 8 h on of dialysis. Instead, the SPC:CaS formulation presented a value significantly lower of %EE from 3 h on of dialysis. Besides, of the three systems, SPC:SA was the formulation that gave the higher encapsulation efficiency in the dialysis. This result could be related to the greater membrane rigidity in this formulation with FA (Table 1, GP value).
It should be noted that at the end of the dialysis, when the liposomes were disruptured after 72 h, the values of EE% showed that the three systems retained a high percentage of FA without significant difference between them (Tukey test, statistic not shown in Table 2).