► Aspergillus lentulus displayed high MIC and varied toxicity response against various metals. ► Significant removal of Cr (79%), Cu (78%), Ni (42%) & Pb (71%) from synthetic solutions. ► Very high uptake capacity for Cr (331 mg g−1) and Pb (1120 mg g−1). ► Simultaneous removal of Cr, Cu & Pb from industrial effluent via bioaccumulation. ► A. lentulus bears high potential for hazardous waste treatment.