2.5.4. Ferric reducing/antioxidant power (FRAP)
The total reducing capacity was determined using the FRAP assay
(Benzie & Strain, 1996). The FRAP reagent was initially prepared
consisting of 300 mM acetate buffer, pH 3.6, 10 mM iron
reagent (TPTZ) solution in 40 mM HCl, and 20 mM FeCl3 6H2O
solution. The fresh working solution was warmed at 37 C before
using. Fruit extracts (100 ll) were allowed to react with 1.9 ml of
the FRAP solution. After incubation for 4 min, the absorbance
was read at 593 nm using a spectrophotometer. The results were
calculated by standard curves prepared with known concentrations
of FeSO4, and were expressed as lmol FeSO4/g.
2.5.4. Ferric reducing/antioxidant power (FRAP)The total reducing capacity was determined using the FRAP assay(Benzie & Strain, 1996). The FRAP reagent was initially preparedconsisting of 300 mM acetate buffer, pH 3.6, 10 mM ironreagent (TPTZ) solution in 40 mM HCl, and 20 mM FeCl3 6H2Osolution. The fresh working solution was warmed at 37 C beforeusing. Fruit extracts (100 ll) were allowed to react with 1.9 ml ofthe FRAP solution. After incubation for 4 min, the absorbancewas read at 593 nm using a spectrophotometer. The results werecalculated by standard curves prepared with known concentrationsof FeSO4, and were expressed as lmol FeSO4/g.
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