MRSA forms biofilms on implants, medical supplies, or on medical devices, which eventually increases the bacterium's antibiotic resistance. In this study, we examined whether an ethanol extract of A. princeps was able to inhibit the biofilm formation of MRSA. Using safranin staining, the ethanol extract of A. princeps at concentrations of 2–8 mg/mL was seen to inhibit MRSA biofilm formation (Figure 2). The positive control used in this study, 0.1% NaF, also inhibited biofilm formation. These results were confirmed by SEM images (Figure 3), which showed similar outcomes to the results of safranin staining. In the control that was not treated with the ethanol extract of A. princeps, MRSA adhered densely to the surface of the polystyrene 35 mm dish and formed biofilms, whereas biofilm formation decreased in proportion to the ethanol extract concentration when the extract was introduced. Biofilm formation also decreased in the positive control.
MRSA forms biofilms on implants, medical supplies, or on medical devices, which eventually increases the bacterium's antibiotic resistance. In this study, we examined whether an ethanol extract of A. princeps was able to inhibit the biofilm formation of MRSA. Using safranin staining, the ethanol extract of A. princeps at concentrations of 2–8 mg/mL was seen to inhibit MRSA biofilm formation (Figure 2). The positive control used in this study, 0.1% NaF, also inhibited biofilm formation. These results were confirmed by SEM images (Figure 3), which showed similar outcomes to the results of safranin staining. In the control that was not treated with the ethanol extract of A. princeps, MRSA adhered densely to the surface of the polystyrene 35 mm dish and formed biofilms, whereas biofilm formation decreased in proportion to the ethanol extract concentration when the extract was introduced. Biofilm formation also decreased in the positive control.
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