Based on this information, we examined Noc-treated cells with a bud/mother size ratio of 0.62 for clustering of mCherry-Cse4. Strikingly, we found that 39% of all Noc-treated cells had an average bud/mother size ratio of 0.72 (SD 0.09) and nonclustered mCherry-Cse4 in the mother with no signal in the daughter.