The channels on the PDMS plate were cut so that they
directly corresponded to the detection region as noted in Fig. 3
and were used to hold the reaction solution. The PC plates
were treated in a UV/ozone cleaner for 20 min to improve the
surface wettability; all three plates were then pieced together
to form a seal, thereby preventing solution leakage. The
barcode was completed by aligning the printed barcode
directly above the PDMS channels.