An aliquot of FE fraction was exposed to extreme heat, 100 _C for 20 min in a hot air oven and allowed to cool to room temperature.Another aliquot of FE was exposed to solar radiation for a period of 15 days in glass beakers. The heat-treated sample is referred as HT and solar radiation exposed sample is referred as NC hereafter for convenience. All the three samples namely, FE, HT and NC were tested for their toxicity on A. albopictus as illustrated in thebioassay procedure below and their toxicity was compared.