Basic genetic parameters including allele frequencies, observed (No)
and effective number of alleles (Ne), observed (Ho) and expected
heterozygosity (He) and heterozygote deficit (FIS) in thewhole population
were calculated by analyzing the genetic data with GenAlEx 6.2
software (Peakall and Smouse, 2008). Tests ofHardy–Weinberg equilibrium
and Ewens–Watterson Neutrality were applied using POPGENE
1.31 version (Yeh et al., 1999). Bottleneck events in the population
were tested by three methods. The first method consisted of three excess
heterozygosity tests developed by Cornuet and Luikart (1996):
(i) Sign test, (ii) Standardized differences test, and (iii) a Wilcoxon