QTL mapping
To map resistance loci in BC1 population of NCEBR2 ×
NC84173, 70 simple sequence repeat (SSR), 23 cleaved
amplified polymorphic sequence (CAPS), 1 insertiondeletion
polymorphism (INDEL), and 5 resistant gene
anologs (RGA) marker were tested to find polymorphism
between the parents. Among tested 99 markers, only 11
markers (11%) were identified as polymorphic between
the parents (Data not shown). Hence, the established
map consisted of 11 markers covering entire haploid
tomato genome (1409 centi Morgan: cM) with 128 cM
intervals.
The SSR polymophic markers between the parents
were located on the following chromosomes: the
TOM196 was on chromosomme 1, the SSR22 was on
chromosomme 2, the SSR63 was on chromosomme 8,
SSR383 was on chromosomme 9, SSR318 and SSR248
were on chromosome 10 and the TOM144 was on
chromosome 11, respectively. The CAPS markers
located as: LEOH18 was on chromosome 4 and RX3-L1
was on chromosome 5. However, CAPS marker LEOH57
and INDEL marker CT107371 found in multiple gene
families could not be mapped to a specific
chromosomme.
QTL mappingTo map resistance loci in BC1 population of NCEBR2 ×NC84173, 70 simple sequence repeat (SSR), 23 cleavedamplified polymorphic sequence (CAPS), 1 insertiondeletionpolymorphism (INDEL), and 5 resistant geneanologs (RGA) marker were tested to find polymorphismbetween the parents. Among tested 99 markers, only 11markers (11%) were identified as polymorphic betweenthe parents (Data not shown). Hence, the establishedmap consisted of 11 markers covering entire haploidtomato genome (1409 centi Morgan: cM) with 128 cMintervals.The SSR polymophic markers between the parentswere located on the following chromosomes: theTOM196 was on chromosomme 1, the SSR22 was onchromosomme 2, the SSR63 was on chromosomme 8,SSR383 was on chromosomme 9, SSR318 and SSR248were on chromosome 10 and the TOM144 was onchromosome 11, respectively. The CAPS markerslocated as: LEOH18 was on chromosome 4 and RX3-L1was on chromosome 5. However, CAPS marker LEOH57and INDEL marker CT107371 found in multiple genefamilies could not be mapped to a specificchromosomme.
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