Using whole soybean for tofu making can effectively reduce the okara waste, increase the utilization of
raw material, and lower the production cost. However, the fiber in whole soybean would alter the protein
concentration in soymilk and might weaken the structure of tofu. In the current study, UHPH was used
for reducing the particle size of soy flour, non-thermally denaturing the soy proteins, and making a high
quality tofu. Two soy flour suspension concentrations (15% and 20%) were treated by UHPH with four
combinations of pressures and cycles. Glucono-d-lactone (GDL) was used as coagulant for tofu making.
Soy flour suspensions heated at 95 C for 10 min were used as the control. The result showed that the
UHPH treated soy flour suspension had a smaller and more uniform particle size than in the control.
Under appropriate pressure and cycles the hardness, gumminess and chewiness of tofu made from soy
flour treated with UHPH was similar to the control tofu. The tofu made from 20% soy flour suspension
with 150 MPa UHPH for 3 cycles had the lowest expressible water and syneresis. Control tofu showed
a particles inlaid, incomplete honeycomb-like network, whereas UHPH tofu had a regular, continuous
honeycomb-like structure. The above results indicated that UHPH reduced the particle size of soy flour,
denatured the soy proteins, and could produce a favorable tofu with high level of functional components
such as fiber